Assistant researcher for vascular biology - Hiring in process/Finished, not possible to apply

Description of the workplace

The research group for "Vascular Biology" conducts research in sphingosine-1-phosphate signaling during cardiovascular diseases. The group is led by Anja Meissner and consists of researchers and the student. The operation is located at the Biomedical Center in Lund. Here you can read more about our exciting research: https://portal.research.lu.se/portal/en/organisations-researchgroups/vascular-biology(6782dcc5-4496-437c-ae27-b5d8db6aa68c).html

Within the research group, we promote a good working environment built on respect and consideration for each other.

To expand our team, we are looking for a researcher to work on sphingosine-1-phosphate signaling during cardiovascular disease.

The desired starting date is 01.06.2022 or by agreement.

We offer

Lund University is a government agency, which means that you receive special benefits, generous
holidays and a favorable occupational pension. Read more on the university's website about being
employed at Lund University. Work with us.

Work duties and area of responsibilities

The candidate is responsible for the investigation of cellular signaling pathways and pathophysiological mechanisms, underlying pathogenic changes in the brain during cardiovascular diseases (especially during high blood pressure, heart failure or stroke). To identify potential target structures for new therapies, a variety of in vivo and ex vivo methods are used.

Your duties mainly include:

• Independent execution of research projects in the field of cardiovascular research with focus on neurological impairment (especially in hypertension, heart failure and stroke models),
• in vivo work in mouse models (incl. surgical induction of stroke and/or heart failure, osmotic pump implantation, various treatment routines), and
• in vivo imaging (MRI), and
• behavioral analysis (neurological tests, cognitive tests incl. NOR etc.),
• molecular, histological and immunological experiments in tissue from mouse models and humans, and
• in vitro experiments involving a set of molecular, histological and immunological techniques.

Qualifications

Requirements

• University degree (MSc) in biochemistry or medicine.
• Doctoral degree in the field of molecular medicine, biochemistry or physiology.
• Previous practical experience in the work with research animals (especially with genetically modified models and mouse models of heart or brain diseases - specifically with models of hypertension, heart failure and stroke).
• Proven experience in performing microsurgical procedures in vivo (e.g., occlusion of small blood vessels in the brain and hearts of mice (e.g., pMCAo or tMCAo, coronary artery ligation) and ex vivo isolation of small blood vessels, and blood vessel tissue fractionation.
• Previous practical experience with behavioral testing and analysis (neurological tests, cognitive tests, etc.) of small research animals (i.e., mice).
• Previous experience with confocal microscopy, live cell images, in vivo imaging methods (MRI techniques).
• Proven experience with S1P signaling (e.g., with mass spectrometry analysis of S1P in plasma and tissue)
• Knowledge in research on cardiovascular diseases (especially in the area of hypertension, heart failure and/or stroke).
• Knowledge of and previous practical experience with RNA scope, RiboTag mouse lines and flow cytometry.
• Knowledge and proven ability to independently perform extensive ex vivo and in vitro experiments and analyses (incl. various protein purification methods).
• Good English skills (writing and writing)
• Collaborative thinking and team spirit.
• Flexibility

Desirable

• Knowledge of and previous practical experience with standard light field and fluorescence microscopy techniques and histology.
• Previous practical experience with techniques such as Western blotting, qPCR, ELISA, immunofluorescence, immunohistochemistry and cell culture (e.g., FPLC, isoelectric focusing; radiolabeling, RNA protein extraction, molecular cloning, transfection, reporter analysis, etc.).
• Previous experience working with neurons, astrocytes or microglia in vivo and/or ex vivo and isolation of these cell types (e.g., practical experience in the work with cell isolation from tissue and with primary cell and/or tissue cultures).
• Practical knowledge in the field of lipid / sphingolipid analysis and their extraction from complex tissue.
• Proven practical experience of working with human tissue.

Other information

The employment is a fulltime, fixed-term position, during a period of one year. Preliminary starting date is 2022-06-01 or according to agreements.

This role includes project-specific mobility required between Germany and Sweden.

How to apply

The application must contain a personal letter in which you describe how you meet the desired qualifications, a CV and your diplomas.

Lund University welcomes applicants with different backgrounds and experiences. We see equality and diversity as a strength and asset. Welcome with your application!