Doctoral student in biochemistry - Hiring in process/Finished, not possible to apply

Subject description

Biophysical chemistry involves research directed towards molecular questions in biological systems. The experimental and theoretical methods used often have their origins in physics. The biophysical chemistry research within the divisions of Physical Chemistry and Biochemistry and Structural Biology (Department of Chemistry), include experimental and theoretical research on soft and biological matter. Significant work is devoted to protein self-assembly and co-assembly and interactions with other biomolecules.

The current project is focussed on a class of chaperone proteins that through interactions increase the solubility of other proteins (clients) and thus prevent precipitation, often through amyloid fibril formation. The aim is to understand the fundamentals of chaperone self-assembly as well as chaperone-client interactions and co-assembly. The specific aims include determination of size-distributions, partition coefficients, stoichiometries, equilibrium versus kinetic traps, energy barriers, and elucidation of co-assembly structures over a range of length scales.

The PhD student will be part of a collaborative network of students and researchers at the divisions of Physical Chemistry (http://www.physchem.lu.se) and Biochemistry and Structural Biology (http://www.cmps.lu.se/biostruct/) at Lund University. The PhD student will be admitted in biochemistry.

Work duties

The project includes experimental studies of chaperone self-assembly and chaperon-client interactions and co-assembly with amyloid forming proteins. The aim is to characterize and understand the relevant protein-protein interactions involved, and the origin of client solubility enhancement at the molecular level. The studies will involve different chaperones and client amyloid proteins. The experimental studies will include the use of several state-of-the art techniques. Relevant experimental methods that are available include optical and NMR spectroscopy, calorimetry, neutron, X-ray and light scattering techniques, fluorescence correlation spectroscopy, surface plasmon resonance and other surface techniques, as well as optical and electron microscopy. The PhD student will execute planning, methodology development, experimental work and data analysis. The detailed direction of the research studies, including the choice of experimental techniques and approaches will be decided together with the supervisors.

The main duties of doctoral students are to devote themselves to research studies, which includes participating in research projects and third cycle courses. The work duties will also include teaching and other departmental duties (not more than 20%).

Admission requirements

A person meets the general admission requirements for third-cycle courses and study programmes if he / she /they:

• has been awarded a second-cycle qualification, or
• has satisfied the requirements for courses comprising at least 240 credits of which at least 60 credits were awarded in the second cycle, or
• has acquired substantially equivalent knowledge in some other way in Sweden or abroad.

Additional requirements:

• Very good oral and written proficiency in English.
• Good knowledge and skills relevant to the thesis project and the work duties.
• Ability to work independently and to formulate and tackle research problems.

Assessment criteria

Selection for third-cycle studies is based on the student’s potential to profit from such studies. The assessment of potential is made primarily on the basis of academic results from the first and second cycle. Special attention is paid to the following:

Knowledge and skills relevant to the thesis project and the work duties. An assessment of ability to work independently and to formulate and tackle research problems. Written and oral communication skills Other experience relevant to the third-cycle studies, e.g. professional experience of biophysical studies of proteins.

Other assessment criteria:

Documented experience of scientific experimental studies of self- and co-assembly processes in lipid, peptide and/or protein systems is a great advantage, as well as both hand-on and theoretical knowledge of one or several of the experimental methods mentioned above.

Consideration will also be given to good collaborative skills, drive and independence, and how the applicant, through his or her experience and skills, is deemed to have the abilities necessary for successfully completing the third cycle programme.

Terms of employment
Only those admitted to third cycle studies may be appointed to a doctoral studentship. Doctoral studentships are regulated in the Higher Education Ordinance (1993:100), chapter 5, 1-7 §§.